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. 2017 May 11;14(6):2147–2157. doi: 10.1021/acs.molpharmaceut.7b00308

Figure 1.

Figure 1

Methotrexate uptake in ciPTEC-OAT1 and ciPTEC-OAT3. Methotrexate (MTX) significantly reduced the uptake of fluorescein (A) and inhibited the efflux of Hoechst33342, in ciPTEC-OAT1 (B) and GS-MF (C), in both ciPTEC-OAT1 and OAT3. Calcein efflux was not blocked by methotrexate in both cell lines (D), and Hoechst33342 activity in ciPTEC-OAT3 could not be determined. Model inhibitors Probenecid, KO143, MK571, and PSC833 were tested as positive controls. Data are presented as mean values ± SEM. Statistical analysis was performed via unpaired Student’s t test. ∗, p < 0.05 and ∗∗∗, p < 0.01 compared to control (CTRL). A schematic depiction of the potential interactions (E).