Figure 10. Mouse TOMT and TMC1 can interact in HEK 293 cells.
Top labels: transfected proteins for all blots. Blue labels: HA-tagged proteins; Burgundy: TMC1; Green: GFP. (A) Anti-HA blot of totals (40% loaded relative to immunoprecipitates). (B) Anti-TMC1 blot of totals. (C) Anti-HA blot of HA immunoprecipitates. (D) TMC1 blot of HA immunoprecipitates. (E) TMC1 blot of GFP immunoprecipitates (immunoprecipitating GFP-TMC1). (F) HA blot of GFP immunoprecipitates. In both HA and GFP immunoprecipitation experiments, a robust interaction was detected between TOMT and TMC1; the H183A change in TOMT enhances this interaction. The HA-tagged controls COMT, EZR, and PRKAR1A did not interact with TMC1-GFP.
Figure 10—figure supplement 1. TOMT-HA and TMC1-GFP in HEK 293 cells.
(A) TOMT-HA only; (B) TMC1-GFP only; (C) TOMT-HA (magenta) and TMC1-GFP (green) co-expressed. Both TOMT and TMC1 were confined to intracellular membranes. Scale bar = 5 µm, applies to A-C.