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. 2017 May 15;6:e24318. doi: 10.7554/eLife.24318

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© 2017, Cunningham et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 6. — (A,B) P6 cochlear inner hair cells from (A) Tomt^add/+ and (B) Tomt^add/add mice stained with anti-Cdh23 (Siemens et al., 2004) and phalloidin. (C,D) P7 cochlear inner hair cells from (C) Tomt^add/+ and (D) Tomt^add/add mice stained with anti-PCDH15-CD2 (Webb et al., 2011) and phalloidin. (E,F) P6 cochlear inner hair cells from (E) Tomt^add/+ and (F) Tomt^add/add mice stained with anti-PMCA2 (Abcam) and phalloidin. (G,H) P8 cochlear inner hair cells from (G) Tomt^add/+ and (H) Tomt^add/add mice stained with anti-MYO7A (Proteus) and phalloidin. (I,J) P8 cochlear inner hair cells from (I) Tomt^add/+ and (J) Tom ^add/add mice stained with anti-Espin (BD Biosciences) and phalloidin. (K,L) P7 cochlear inner hair cells from (K) Tomt^add/+ and (L) Tomt^add/add mice stained with anti-Whirlin (see Materials and methods) and phalloidin. Scale bar in (A) = 5 µm, and applies to (A–L). (M) Tip links from middle region cochlear hair cells from P7-8 Tomt^add/add mice using SEM. Left and middle panels are from inner hair cell, right panel is from outer hair cell. Left panel scale bar = 500 nm, middle panel scale bar = 100 nm and applies to middle and right panels. (N) Quantification of tip-links per stereociliary column from P7-P8 middle region inner and outer hair cells from Tomt^add/+ (IHC n = 10 cells, OHC n = 9 cells) and Tomt^add/add (IHC n = 17 cells, OHC n = 9) mice.

DOI: http://dx.doi.org/10.7554/eLife.24318.009

Figure 6—figure supplement 1. — (A,B) P6 cochlear inner hair cells from (A) Tomt^add/+ and (B) Tomt^add/add mice stained with anti-Cdh23 (Siemens et al., 2004) and phalloidin. (C,D) P7 cochlear inner hair cells from (C) Tomt^add/+ and (D) Tomt^add/add mice stained with anti-PCDH15-CD2 (Webb et al., 2011) and phalloidin. (E,F) P6 cochlear inner hair cells from (E) Tomt^add/+ and (F) Tomt^add/add mice stained with anti-PMCA2 (Abcam) and phalloidin. (G,H) P8 cochlear inner hair cells from (G) Tomt^add/+ and (H) Tomt^add/add mice stained with anti-MYO7A (Proteus) and phalloidin. (I,J) P8 cochlear inner hair cells from (I) Tomt^add/+ and (J) Tom ^add/add mice stained with anti-Espin (BD Biosciences) and phalloidin. (K,L) P7 cochlear inner hair cells from (K) Tomt^add/+ and (L) Tomt^add/add mice stained with anti-Whirlin (see Materials and methods) and phalloidin. Scale bar in (A) = 5 µm, and applies to (A–L). (M) Tip links from middle region cochlear hair cells from P7-8 Tomt^add/add mice using SEM. Left and middle panels are from inner hair cell, right panel is from outer hair cell. Left panel scale bar = 500 nm, middle panel scale bar = 100 nm and applies to middle and right panels. (N) Quantification of tip-links per stereociliary column from P7-P8 middle region inner and outer hair cells from Tomt^add/+ (IHC n = 10 cells, OHC n = 9 cells) and Tomt^add/add (IHC n = 17 cells, OHC n = 9) mice.

DOI: http://dx.doi.org/10.7554/eLife.24318.009