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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Cell Physiol. 2017 Mar 27;232(9):2447–2460. doi: 10.1002/jcp.25582

Figure 1.

Figure 1

Expression and distribution of VLK in human TM cells, tissue and aqueous humor. Confirmation of VLK expression in human TM cells (derived from two different donor eyes-lanes 1 & 2) by RT-PCR amplification (A). While VLK specific DNA products of the expected size were amplified with primer sets (P) 3, 4 & 5, no DNA product was detected using set 1 and 2 primers. Lane M depicts nucleotide bp markers; –RT; sample derived from the absence of reverse transcriptase. B. Immunoblotting analysis of VLK protein in TM cell lysates and conditioned media (CM) derived from three different samples (lanes 1–3) using VLK polyclonal antibody identified a prominent immunopositive species of approximately 32 kDa in both lysates and CM. C. Distribution of VLK (red) in TM cells by immunofluorescence shows a Golgi/endoplasmic reticulum specific localization (white arrows). D. Immunofluorescence-based analysis of VLK distribution in the human AH outflow pathway (paraffin sections) shows positive staining in the TM, and the Schlemm’s canal (SC) and juxtacanalicular area (JCT). In panels C & D, the nuclei were stained with Hoechst 33342 (blue) and bars indicate image magnification. E. Detection of VLK in the AH of human and porcine by immunoblot analysis.