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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Cell Physiol. 2017 Mar 27;232(9):2447–2460. doi: 10.1002/jcp.25582

Figure 3.

Figure 3

RhoA activation and cyclic mechanical stretch increase VLK protein levels in human TM cell conditioned media. TM cells expressing a constitutively active RhoA (RhoAV14 mutant) for 36 h under serum free conditions exhibited significantly increased levels of VLK protein in the CM based on immunoblot analysis and densitometric quantification as compared to GFP expressing control cells (B&D). N=7, *P<0.05. VLK protein levels in TM cell lysates however, were found to be unaltered in cells expressing RhoAV14 (A&C; n=7). Lower panels in A & B show the loading controls. E& F. Subjecting TM cells (lanes 1–3 represent three independent samples) grown on type 1 collagen-coated silicone sheets to cyclic mechanical stretch (20% stretching, one cycle per second) for 48 h significantly increased VLK protein levels in the CM compared to control cells based on immunoblotting and densitometric analyses. N=6, *P<0.05. Loading control is shown in the lower panel of E. Values are mean ± SEM.