Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Cell Physiol. 2017 Mar 27;232(9):2447–2460. doi: 10.1002/jcp.25582

Figure 5.

Figure 5

VLK deficiency suppresses TyrP of ECM proteins secreted by human TM cells. A. The ECM-enriched extracellular protein fraction (SDS-urea soluble) derived from TM cells (lanes 1–4 represent 4 independent samples) treated with scrambled siRNA (S siRNA) for 72 h showed an intense immunostaining for TyrP proteins with molecular mass ranging from 50 to >250 kDa in immunoblots developed using MultiMab phospho-Tyrosine rabbit mAb. In contrast to the scrambled siRNA treated cells, TM cells treated with VLK-specific siRNA (V siRNA) demonstrated a dramatic and significant decrease in the levels of TyrP ECM proteins based on immunoblot quantification (A&B). Panels C and D show loading controls. Values are mean ±SEM of 8 independent samples. *P<0.05.