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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Cell Physiol. 2017 Mar 27;232(9):2447–2460. doi: 10.1002/jcp.25582

Figure 7.

Figure 7

Suppression of TGF-β2 induced TyrP of ECM proteins by VLK deficiency in TM cells. A. TGF-β2 induced TyrP of ECM proteins (SDS-urea soluble fraction) derived from the human TM cell cultures. Serum starved TM cells were treated with TGF-β2 (4 ng/ml) for 48 h and ECM enriched fraction was isolated as described in Fig. 6B. ECM enriched fractions were analyzed for changes in TyrP by immunoblotting and densitometric analysis. TGF-β2 treated samples (10 μg) showed a significant increase in the levels of TyrP of ECM proteins (prominent species indicated by arrows in panel A) as compared to control cells (B). Values represent the mean ±SEM of 4 independent analyses. C & D. Compared to TM cells treated with scrambled siRNA alone or together with TGF-β2, cells treated with VLK siRNA in the absence or presence of TGF-β2 showed a significant decrease in the levels of TyrP ECM proteins. Panel E shows a loading control. Values are mean ±SEM of 8 independent analyses. *P<0.05.