Figure 2.

In vitro splenocytes proliferation response in mice. Spleen lymphocytes were harvested from mice immunized with pcGRA2, pcGRA5, pcDNA 3.1C, and phosphate-buffered saline (PBS) 3 weeks after last injection. The splenocytes were cultured and stimulated with total lysate antigen. Proliferative response was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data are expressed as mean stimulation index (SI) ± standard deviation (N = 3). Statistical difference is represented by * (P < 0.05) in comparison with the control groups (PBS or pcDNA 3.1C).