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. 2017 Jun 7;96(6):1441–1447. doi: 10.4269/ajtmh.16-0548

Figure 3.

Figure 3.

Interferon-gamma (IFN-γ) and interleukin-2 (IL-2) production by the stimulated splenocytes of the immunized mice. Culture supernatants from the total lysate antigen–stimulated immunized mice splenocytes were collected at 96 and 24 hours postincubation for the evaluation of (A) IFN-γ and (B) IL-2 production, respectively, via enzyme-linked immunosorbent assay. Data are expressed as mean ± standard deviation (N = 3). Statistical difference is represented by * (P < 0.05) in comparison with the control groups (phosphate-buffered saline [PBS] or pcDNA 3.1C).