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. Author manuscript; available in PMC: 2018 Apr 7.
Published in final edited form as: J Proteome Res. 2017 Mar 8;16(4):1763–1772. doi: 10.1021/acs.jproteome.7b00024

Figure 1.

Figure 1

Workflow of mass spectrometry identification and quantitation of exosome proteins from patient serum. Schematic overview of the sample preparation and LC–MS/MS analysis of patient and control serum samples. Exosomes were isolated from sera of patients with pancreatic cancer using several steps of differential centrifugation. Then, proteins were extracted, alkylated, and digested in ultracentrifugal tubes using the filter-aided sample preparation (FASP) method. Next, peptides were labeled with isobaric tags for relative and absolute quantitation (iTRAQ). Next, all plex of iTRAQ-labeled peptides were mixed and run on a mass spectrometer. Finally, database search of raw data and statistical analysis identified the differentially expressed proteins.