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. Author manuscript; available in PMC: 2018 Apr 7.
Published in final edited form as: J Proteome Res. 2017 Mar 8;16(4):1763–1772. doi: 10.1021/acs.jproteome.7b00024

Figure 4.

Figure 4

Tumor-derived exosomes increase the migration potential of pancreatic cancer cell lines. To evaluate changes in migration potential, Panc-1 and MiaPaCa2 cells were exposed to the (a) serum of 5 healthy controls, (b) the serum of four patients with locally advanced pancreatic cancer, (c) exosome-depleted serum, or (d) purified exosomes in a wound healing assay. Wound closure was evaluated 0, 12, and 24 h after wound induction. While treatment with (a) serum derived from healthy controls had no impact on migration in either cell line, (b) the migration potential was increased in cells treated with serum from patients 2, 3, and 4 but not patient 1. (c) Depletion of exosomes from patient serum negated the pro-migratory effects of patient serum. (d) Pro-migratory effects of patient serum can be attributed to serum exosomes and are preserved in purified exosomes. Representative images of Panc-1 cells treated with (e) exosome-depleted serum or (f) purified exosomes of a healthy control and a patient with locally advanced pancreatic cancer. Error bars represent SD (n = 3).