Figure 4.

The Effect of Spatially Patterned Stimuli on Phase Shifting

(A) Double-plotted actograms and phase shift of the same mouse to different light pulse stimuli. Mice were pulsed at CT 16 with either a grating- or an irradiance-matched spatially uniform gray screen.

(B) Stimulus set up for the light pulse. Mice were placed in a glass area surrounded by four monitors.

(C) Irradiance response curve for both spatially uniform stimuli (filled circles) and drifting sinusoidal gratings (0.03 cpd; 4 Hz; open circles). Maximum irradiance was 8.7 × 10¹³ total photons.cm⁻².s⁻¹. No significance was observed between curves (linear p = 0.89; sigmoidal p = 0.99; graph plotted with sigmoidal function of pooled data).

(D) Paired t test for intra-individual responses between a static square wave grating (0.03 cpd) and a uniform stimuli at both the irradiance that produced the half-maximal (8.6 × 10¹¹ total photons.cm⁻².s⁻¹) and maximal (6.4 × 10¹³ total photons.cm⁻².s⁻¹) response. No difference between the spatial patterned stimuli and the uniform stimuli was detected at either irradiance (half maximum: p = 1.00; maximal: p = 0.78).