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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Proteomics. 2016 Dec;16(23):3042–3053. doi: 10.1002/pmic.201600057

Figure 5. U1 snRNP components correlate with Aβ detergent insolubility and aggregate in AsymAD cases (.

Figure 5

A) Relative quantification of U1-70K (left), U1A (middle) SmD (right) in the detergent-insoluble proteome across individual control, AsymAD, MCI and AD cases. (B) Strong correlation was observed between Aβ and the U1 snRNPs U1-70K, U1A and SmD across all 35 samples analyzed by label-free MS quantification. Log2-transformed quantification data (signal/noise), centered on the geometric mean is plotted on the y-axis and each individual case on the x-axis. (C) Western blot analysis of two AsymAD cases with high levels of U1-70K (left lanes) and two AsymAD cases that had low levels of U1-70K (middle lanes). Two AD cases served as positive control of U1-70K enrichment (right lanes). Calnexin was used as a loading control (bottom). (D) Double-labeled immunofluorescence of cells in AD prefrontal cortex displaying cytoplasmic aggregates of U1-70K alone (green, arrow), tau alone (red, arrowhead), and both (asterisk). Hoescht dye was used to stain nuclei (blue). Scale bar is 20 μm.