Fig. 3.

Spatial and temporal variation in vector competence of wild C. pipiens biotype pipiens and C. torrentium populations.

(A–C) For comparison of infection rates between two years and two geographically distinct mosquito populations of the same species, the data obtained through infection experiments described in Table 1 and Fig. 1 of 2013 were analysed together with data obtained in 2012.

(A) Infection rates for 2012 are indicated by dark grey bars and infection rates for 2013 are represented by light grey bars. Statistical analysis was performed using GraphPad Prism and Fisher's exact test (p < 0.05). Detailed numbers of individuals and numbers of independent experiments used for analysis are listed in Table 1.

(B and C) Infection rates are determined via WNV-specific qRT-PCR (cut-off = 35 cycles). Statistical analysis was performed using GraphPad Prism and Fisher's exact test (p < 0.05). Detailed numbers of individuals and numbers of independent experiments used for analysis are listed in Table 1.

(B) Infection rates for C. pipiens biotype pipiens at 21 days post-infection and two temperatures, 18 °C and 25 °C, for the North population (N) and South population (S) respectively.

(C) Infection rates for C. torrentium at 21 days post-infection and two temperatures, 18 °C and 25 °C, for the North population (N) and South population (S) respectively.