Table 1.
Summary of mosquito samples used for WNV infection assays in 2013 and 2012.
The following lab strains were kept in captivity for 2 month to 2 years prior to the WNV infection assay: Culex quinquefasciatus (C. qui; Malaysia strain, Bayer Company), Culex pipiens biotype molestus Heidelberg (C. Mol S), Culex pipiens biotype molestus Wendland (C. Mol W) and Culex pipiens biotype molestus Langenlehsten (C. Mol LL).
The following species were reared from field-collected egg rafts: Culex pipiens biotype pipiens from Hamburg/Langenlehsten/Altes Land (C. pip North), Culex pipiens biotype pipiens from Lake Constance (C. pip South), Culex torrentium from Hamburg/Langenlehsten/Altes Land (C. tor North) and Culex torrentium from Lake Constance (C. tor South).
For each infection time point, the numbers of individual blood feeding assays performed (experiments) are listed. In addition, the combined number of mosquitoes presented with an infected blood meal during these blood meal assays (individuals) is also depicted.
| Species | Origin | Egg rafts | Temperature [°C] | Infection time (days) | Individuals [#] | Experiments [#] |
|---|---|---|---|---|---|---|
| C. qui | Malaysia | Lab strain | 25 | 14 | 66 (88) | 9 (4) |
| 25 | 21 | 169 (88) | 12 (4) | |||
| 25 | 28 | 41 | 5 | |||
| 25 | 35 | 103 | 7 | |||
| 18 | 14 | 29 | 6 | |||
| 18 | 21 | 64 | 6 | |||
| 18 | 28 | 38 | 5 | |||
| 18 | 35 | 36 | 5 | |||
| C. pip | North | 186 (222) | 25 | 14 | 22 (94) | 5 (3) |
| 25 | 21 | 65 (173) | 7 (3) | |||
| 25 | 28 | 53 | 6 | |||
| 25 | 35 | 69 | 5 | |||
| 18 | 14 | 23 | 5 | |||
| 18 | 21 | 41 | 5 | |||
| 18 | 28 | 46 | 5 | |||
| 18 | 35 | 72 | 5 | |||
| C. pip | South | 311 | 25 | 14 | 8 | 2 |
| 25 | 21 | 24 | 4 | |||
| 25 | 28 | 11 | 3 | |||
| 25 | 35 | 10 | 3 | |||
| 18 | 14 | 8 | 2 | |||
| 18 | 21 | 26 | 4 | |||
| 18 | 28 | 12 | 3 | |||
| 18 | 35 | 14 | 3 | |||
| C. mol | S, W, LL | Lab strains | 25 | 14 | 80 | 6 |
| 25 | 21 | 273 | 14 | |||
| 25 | 28 | 109 | 11 | |||
| 25 | 35 | 90 | 6 | |||
| 18 | 14 | 15 | 3 | |||
| 18 | 21 | 24 | 4 | |||
| 18 | 28 | 39 | 5 | |||
| 18 | 35 | 67 | 5 | |||
| C. tor | North | 225 (119) | 25 | 14 | 12 (130) | 3 (4) |
| 25 | 21 | 34 (138) | 6 (4) | |||
| 25 | 28 | 9 | 3 | |||
| 25 | 35 | 9 | 3 | |||
| 18 | 14 | 12 | 3 | |||
| 18 | 21 | 15 | 3 | |||
| 18 | 28 | 16 | 3 | |||
| 18 | 35 | 19 | 3 | |||
| C. tor | South | 29 | 25 | 14 | 0 | 0 |
| 25 | 21 | 12 | 3 | |||
| 25 | 28 | 3 | 1 | |||
| 25 | 35 | 0 | 0 | |||
| 18 | 14 | 0 | 0 | |||
| 18 | 21 | 7 | 2 | |||
| 18 | 28 | 10 | 2 | |||
| 18 | 35 | 0 | 0 |
The numbers written in parentheses represent the number of individuals/experiments/field-collected egg rafts used for experiments in 2012. All other values are data from 2013.