Table 4.
Purification strategies and biochemical attributes of purified pectin lyases from different microbial sources
| Source organism | Purification strategy | PH optima | pH stability | Temp optima (oC) | Temp stability (oC) | K m (mg/mL) | MW (kD) | Ref. |
|---|---|---|---|---|---|---|---|---|
| Aspergillus niger | FPLC, gel chromatography | 5.0 | 3.0–7.0 | 43 | 25–50 | 0.66 | 47 | (Xu et al. 2015) |
| Fusarium decemcellulare | Ion exchange, gel filtration | 9.0 | 9.0–12.0 | 50 | 10–40 | 0.125 | 45 | (Yadav et al. 2014) |
| Penicilium purpurogenum | Phenyl agarose chromatography | 6.0 | – | 50 | Upto 50 | – | 45 | (Pérez- Fuentes et al. 2014) |
| Aspergillus flavus | Ion exchange, gel filtration | 8.0 | 3.0–11.0 | 55 | 10–40 | 1.70 | 50 | (Yadav et al. 2013) |
| Bacillis clausii | Ion exchange, gel filtration | 10.0 | 8.0–10.0 | 60 | Upto 40 | 0.87 | 35 | (Li et al. 2012) |
| Oidiodendron echinulatum | Ion exchange, gel filtration | 7.0 | Upto 5.0 | 50 | 10–50 | 1.20 | – | (Yadav et al. 2012) |
| Aspergillus giganteus | Ion exchange, gel filtration | 8.5–9.0 | 6.0–10 | 50 | – | – | – | (Pedrolli and Carmona 2014) |
| Aspergillus terricola | Ion exchange, gel filtration | 8.0 | 4.0–9.0 | 50 | 10–50 | 1.0 | 35 | Yadhav et al. (2009) |
| Penicillium citrinum | Gel filtration, ion exchange | 9.0 | 9.0–12.0 | 50 | 10–40 | 1.0 | 31 | Yadhav et al. (2009) |
| Penicillium canescens | Ion exchange, hydrophobic chromatography | 5.0–5.5 | 5.0–6.0 | 60 | Upto 50 | – | 37.5 | (Sinitsyna et al. 2007) |
| Penicillium oxalicum | Ion exchange, gel filtration | 8.0 | – | 50 | – | 1.1 | 50 | (Yadav and Shastri 2007) |
| Cystofolibasidium Capitatum | Ion exchange, hydrophobic interaction chromatography, gel filtration | 8.0 | 3.0–9.0 | 40 | 36.6 | 42 | (Nakagawa et al. 2005) |