Table 1.
Matrix of the PB12 design for selection of the best condition for production of bioherbicide by solid-state fermentation
| Runs | Temperature (°C) | Moisture (wt%) | CSL (wt%) | SB (wt%) | Inoculum (wt%) | Damage of leaves (%) |
|---|---|---|---|---|---|---|
| Water | – | – | – | – | 0 | 0.0 g* |
| T1 | 35 | 50 | 10 | 0 | 5 | 0.36 d |
| T2 | 35 | 75 | 0 | 10 | 5 | 0.28 d |
| T3 | 25 | 75 | 10 | 0 | 15 | 0.14 e |
| T4 | 35 | 50 | 10 | 10 | 5 | 0.89 b |
| T5 | 35 | 75 | 0 | 10 | 15 | 0.62 c |
| T6 | 35 | 75 | 10 | 0 | 15 | 1.01 b |
| T7 | 25 | 75 | 10 | 10 | 5 | 0.04 f |
| T8 | 25 | 50 | 10 | 10 | 15 | 1.23 a |
| T9 | 25 | 50 | 0 | 10 | 15 | 0.94 b |
| T10 | 35 | 50 | 0 | 0 | 15 | 0.52 c |
| T11 | 25 | 75 | 0 | 0 | 5 | 0.24 d |
| T12 | 25 | 50 | 0 | 0 | 5 | 0.25 d |
| T13 | 30 | 62.5 | 5 | 5 | 10 | 0.02 g |
| T14 | 30 | 62.5 | 5 | 5 | 10 | 0.01 g |
| T15 | 30 | 62.5 | 5 | 5 | 10 | 0.01 g |
* Mean followed by the same letter in the column did not differ statistically by the Scott-Knott’s test at 95% of confidence level (p < 0.05)