. 2017 Jun 8;7(2):137. doi: 10.1007/s13205-017-0761-2

Table 1.

Quantitative and qualitative and estimation of siderophores by different tests, HCN production and antagonistic activity of fluorescent Pseudomonas isolates against Rhizoctonia solani and Sclerotium rolfsii

Isolates	Quantitative % siderophore units	Qualitative siderophore test					HCN production	% inhibition
Isolates	Quantitative % siderophore units	CAS	Arnow’s	FeCl₃	Tetrazolium	HQ	HCN production	R. solani	S. rolfsii
Fluorescent Pseudomonas isolates with high ACC deaminase activity (data not shown)
P66	78.555 ± 0.39^a	63.5 ± 1.5^a		+ve	+ve	+++	–	68.89 ± 1.11^a	82.78 ± 2.78^b
P141	80.15 ± 0.15^a	55 ± 1^b		+ve	+ve	+	–	52.78 ± 2.78^c	43.335 ± 2.22^g
P200	49.475 ± 2.11^f	45.5 ± 1.5^de		+ve	+ve	+	–	60 ± 5.56^b	28.89 ± 1.11ⁱ
P229	22.365 ± 1.32^l	36 ± 1^h				+	–	19.445 ± 2.78^j	28.885 ± 4.45ⁱ
P260	71.05 ± 2.63^b	61.5 ± 0.5^a		+ve	+ve	++	–	37.775 ± 5.56^gh	32.775 ± 3.33^hi
Fluorescent Pseudomonas isolates ineffective against R solani and S. rolfsii
P2	37.25 ± 1.06ⁱ	35 ± 1^h			+ve	+	–	40.5 ± 0.5^fg	17.25 ± 0.55^j
P3	30.2 ± 0.28^j	52.5 ± 2.5^bc			+ve	+	–	32.95 ± 0.45^hi	17.1 ± 0.4^j
P43	70.6 ± 0.85^b	41 ± 1^efg	+ve	+ve	+ve	–	–	26.2 ± 1.2^ij	33.95 ± 0.65^hi
P45	24.15 ± 0.21^l	16.5 ± 1.5ⁱ	+ve		+ve	–	+	13.05 ± 0.55^kl	25.1 ± 0.7ⁱ
P130	47.5 ± 0.32^f	31.5 ± 1.5^hi			+ve	+	–	25.15 ± 0.15^ij	31.85 ± 0.75^hi
Fluorescent Pseudomonas isolates moderately effective against R solani and S. rolfsii
P163	47.5 ± 0.71^f	40 ± 2^fgh			+ve	–	–	21.825 ± 0.58^j	45.15 ± 0.75^g
P123	43.78 ± 0.31^g	39 ± 2^gh		+ve	+ve	++	–	33.15 ± 0.65^hi	34 ± 0.7^h
P150	43.78 ± 0.31^g	42.5 ± 2.5^efg			+ve	⁺	+	38.2 ± 0.7^gh	44.95 ± 0.55^g
P23	48.25 ± 0.35^f	42 ± 2^efg		+ve	+ve	–	–	39.375 ± 0.63^fg	45.05 ± 0.65^g
Fluorescent Pseudomonas isolates effective against S. rolfsii (non HCN producing)
P56	27.5 ± 0.71^k	11.5 ± 1.5ⁱ			+ve	–	–	10.8 ± 0.8^l	50.65 ± 0.65^f
P184	40.615 ± 0.87^h	40.5 ± 0.5^fgh		+ve	+ve	–	–	18 ± 0.5^jk	50.75 ± 0.75^f
P207	36.16 ± 0.23ⁱ	51 ± 1^bc		+ve	+ve	+++	–	46.875 ± 0.63^de	72.8 ± 0.6^c
Fluorescent Pseudomonas isolates effective against S. rolfsii (HCN producing)
P132	23.835 ± 0.23^l	41 ± 1^efg			+ve	+	+	13.15 ± 0.65^kl	61.95 ± 0.85^d
P7	52.9 ± 0.14^e	41.5 ± 1.5^efg	+ve	+ve	+ve	++	+	21.625 ± 0.38^j	61.8 ± 0.7^d
P192	36.115 ± 0.16ⁱ	54.5 ± 1.5^b		+ve	+ve	+++	+	28.2 ± 0.7ⁱ	56.25 ± 0.65^e
P191	47.945 ± 0.08^f	64.5 ± 1.5^a			+ve	+++	+	30.8 ± 0.8ⁱ	72.6 ± 0.4^c
P59	60.5 ± 0.71^c	39.5 ± 1.5^gh		+ve	+ve	+++	+	36.625 ± 0.38^gh	47.3 ± 0.6^fg
P29	60.5 ± 0.71^c	54.5 ± 1.5^b		+ve	+ve	+++	+	43.85 ± 1.35^ef	87.3 ± 0.6^a
P187	60.115 ± 0.16^c	51.5 ± 1.5^bc		+ve	+ve	+++	+	44.325 ± 0.57^ef	74.05 ± 0.75^c
Fluorescent Pseudomonas isolates effective against R solani (non HCN producing)
P74	27.75 ± 0.35^k	40.5 ± 2.5^fgh		+ve	+ve	+	–	50.7 ± 0.7^cd	35.4 ± 0.3^h
P80	32.5 ± 0.71^j	s ± 1.5^def		+ve	+ve	++	–	63.1 ± 0.6^b	63.85 ± 0.55^d
Fluorescent Pseudomonas isolates effective against R solani and S. rolfsii (HCN producing)
P144	43.5 ± 0.71^g	48.5 ± 1.5^cd		+ve	+ve	+++	+	50.7 ± 0.7^cd	76 ± 0.4^c
P166	60.115 ± 0.16^c	40 ± 3^fgh			+ve	+++	+	73.1 ± 0.6^a	73 ± 0.8^c
P174	55.5 ± 0.71^d	51 ± 1^bc		+ve	+ve	+++	+	51 ± 1^cd	82.95 ± 0.75^b
Max.	80.15	64.5						73.1	87.3
Min.	22.365	11.5						10.8	17.1
CV	2.352	5.22						6.625	3.645
CD (0.01)	2.962	6.33						6.876	5.12
CD (0.05)	2.198	4.697						5.102	3.799
F cal	527.375**	53.39**						90.948**	247.998**

Values are average of three replications; values after ± represent standard deviation

As per Duncan’s grouping means with the same letter are not significantly different

CV coefficient of variance, CD critical difference, HQ hydroxyquinoline test

+++ Luxuriant/high growth

++ Medium growth

+ Low growth

– No growth

** Values are significant at 1 and 5% levels