Table 1.
Distribution of animal groups for Garcinia indica rind administration
| Group | Study | Treatment given | Treatment days | Comments |
|---|---|---|---|---|
| I | Preliminary studies | Garcinia indica rind (0.1% to 10% w/w, p.o.) | 7 | To determine submaximal effective concentrations |
| II | Elevated plus maze (EPM) | Normal diet | 14 | Control group |
| III | Diazepam (1 mg/kg, i.p.) | Once, 30 min before observation | Positive control group | |
| IV, V and VI | GI (0.5, 1 and 2% w/w p.o.) | 14 | Treatment group | |
| VII–XI | Light–dark model (LDM) | Same pattern of control, positive control and treatment group as in II–VI | ||
| XII–XVI | Hole-board test (HBT) | Same pattern of control, positive control and treatment group as in elevated plus maze study (group no II–VI) | ||
| XVII | Tail suspension test (TST) | Normal diet | 14 | Control group Reading was taken after 30 min of last treatment |
| XVIII | Fluoxetine (20 mg/kg, p.o.) | 14 | Positive control group | |
| XIX | Imipramine (15 mg/kg, p.o.) | 14 | Positive control group | |
| XX, XXI and XXII | GI (0.5, 1 and 2% w/w, p.o.) | 14 | Treatment group Reading was taken after 30 min of last treatment |
|
| XXIII–XXVIII | Forced swim test (FST) | Same pattern of control, positive control and treatment as in TST study (group no XVII–XXII) | ||
| XXIX–XXXIII | Reserpine-induced hypothermia in rats | Same pattern of control, positive control and treatment as in TST study (group no XVII–XXII) except treatments were given for 8 days | ||
| XXXIV | Locomotor activity | Normal diet | 14 | Control group |
| XXXV–XXXVII | GI (0.5, 1 and 2% w/w, p.o.) | 14 | Treatment group | |
| XXXVIII | Biochemical estimations monoamine oxidase, i.e., MAO-A, MAO-B activity and malondialdehyde (MDA) levels | Normal diet | 14 | Control group |
| XXXIX | Fluoxetine (20 mg/kg, p.o.) | 14 | Positive control group | |
| XL–XLII | GI (0.5, 1 and 2% w/w, p.o.) | 14 | Treatment group | |
| XLIII–XLV | Mechanistic study using TST | Normal diet for 14 days Prazosin (62.5 μg/kg, i.p.)/sulpiride (50 mg/kg, i.p.) on the 14th day and p-CPA (100 mg/kg, i.p.) from the 11th day to the 14th day after 60 min of diet administration |
Negative Controls | |
| Group XLVI–XLVIII | GI (1% w/w) for 14 days Prazosin (62.5 μg/kg, i.p.)/sulpiride (50 mg/kg, i.p.) on the 14th day and p-CPA (100 mg/kg, i.p.) from the 11th day to the 14th day after 30 min of administration of GI selected dose |
Treatment | ||