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. 2017 Jun 7;7:2980. doi: 10.1038/s41598-017-03354-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time PCR validation of exon array data. (A) Selection of 8 splicing probe-sets from non-differentially expressed genes. For these 8 genes/probe-sets, there were no differences in the level of gene expression, but significant differences in the level of probe-set expression. (B) RT-PCR results of the 8 probe-sets. RT-PCR results are represented as scatter dot plots of expression fold change in PDAC compared to normal pancreas, with the significance level shown at the top (* < 0.05, ** < 0.01, *** < 0.001). Corresponding exon array results are reported in histograms of log₂ FIRMA fold change (PDAC/normal) values. The two sets of results corresponded well with each other very well.