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. 2017 Jun 7;7:3007. doi: 10.1038/s41598-017-03020-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M. marinum ΔwhiB4 was attenuated in zebrafish. (A) Zebrafish (15 per group) were infected with indicated strain at 10⁴ CFU bacteria per fish, and were monitored for mortalities over a 40-day period. The survival curves were plotted using the Kaplan-Meier method and differences between curves were analyzed using the log-rank test. All fish infected with ΔwhiB4 or PBS survived. The survival curve of the ΔwhiB4 group was significantly different to those of WT (p = 0.0005) or the complemented strain (p < 0.0001). A similar result was found in another independent experiment (B).