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. 2017 Jun 7;7:3007. doi: 10.1038/s41598-017-03020-4

Figure 4.

Figure 4

M. marinum ΔwhiB4 caused less severe pathology. Histological analysis of fish tissues from the experiment described in Fig. 3A. (A,B) Liver sections of fish infected with WT M. marinum at 3 weeks post-challenge. Samples were analyzed with hematoxylin and eosin (H&E) staining (A) and Ziehl-Neelsen method of acid fast staining (B). Both are 20× magnification. (C) H&E staining of liver sections of fish infected with ΔwhiB4 at 3 weeks post-infection (4× magnification). (D,E) Liver sections of fish infected with ΔwhiB4 at 3 weeks post-infection (10× magnification) and stained with H&E (D) or Ziehl-Neelsen (E). The well-organized granuloma observed in (E, white star) was zoomed in for better visualization (F), 40× magnification.