Figure 2.

PIs impair proliferation and clonogenicity of hMADS cells, chin and Knee APs. (A) Dose response curve for DRV and LPV effect on hMADS cell proliferation. Proliferation was evaluated using the soluble tetrazolium salt MTT reduction assay after 4 days of proliferation in presence of DRV (green dots) or LPV (Blue dots). The results represent the mean ± SEM of three experiments carried out in triplicate. Error bars were omitted when the SEM was smaller than the size of the symbol. (B) Dose dependent effects of DRV and LPV on hMADS cell proliferation. Cells were seeded in 12-well plates and grown for 5 days in complete culture medium and counted using a BeckmanZ1 coulter. The results represent the mean ± SEM of three experiments carried out in triplicate (*p < 0.05). (C) Lopinavir but not Darunavir reduces clonogenicity of hMADS cells. After plating and growing 200 cells for 3 weeks in complete culture medium, the percentage of colonies obtained from hMADS cells was calculated. Mean ± SEM was representative of three independent experiments (*p < 0.05). (D,E) Dose dependent effects of DRV and LPV on knee and chin APs cell proliferation respectively. Cells were seeded in 12-well plates and grown for 5 days in complete culture medium and counted using a BeckmanZ1 coulter. Experiment was carried out using APs derived from the same person. The results represent the mean ± SEM of three experiments carried out in triplicate (*p < 0.05, **p < 0.01, ***p < 0.001). ND means “no significant difference with the control condition”.