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. 2017 Jun 7;7:2939. doi: 10.1038/s41598-017-01153-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-19a contributes to EMT in NSCLC cells (A). A549 cells were treated with gefitinib (5 μM) and then transfected with miR-19a or miR-NC; after 24 h, cell migration was detected with a transwell assay. Migrated A549 cells were stained with crystal violet (left); miR-19a overexpression significantly decreased cell migration; quantified data are shown on the right. **p < 0.01; (B) A549 cells were treated with gefitinib (5 μM) and then transfected with Inh-19a or Inh-NC; after 24 h, cell migration was detected with a transwell assay, and migrated A549 cells were stained with crystal violet (left); miR-19a down-regulation significantly increased cell migration; quantified data are shown on the right. **p < 0.01; (C) A549 cells were treated with gefitinib (5 μM) and then transfected with Inh-19a or Inh-NC; the cell morphology is shown. The Inh-19a group exhibited spindle-shaped cells and loss of cell-cell contact, which suggested EMT; (D) A549 cells were treated with gefitinib (5 μM) and then transfected with Inh-19a or Inh-NC, and EMT pathway markers were detected by western blotting.