Table 2.

Sequences of primers used for estrogen receptor-α genotyping

Polymorphism site	Primer sets	Annealing temperature (°C)	Restriction enzyme	Allele size (bp)
Intron 1 T/C	5'-ctgccaccctatctgtatcttttcctattctcc-3' 5'-tctttctctgccaccctggcgtcgattatctga-3'	64	PvuII	T: 936 + 438 C: 1374
Intron 1 A/G	5'-ctgccaccctatctgtatcttttcctattctcc-3' 5'-tctttctctgccaccctggcgtcgattatctga-3'	64	XbaI	A: 981 + 393 G: 1374
Exon 8 G/A	5'-gaggagacggaccaaagccac-3' 5'-gccattggtgttggatgcatg-3'	63	BtgI	G: 129 + 98 A: 227

bp, base pairs.