Figure 3.

Hydroxyapatite particle shape dictates cytokine production by murine BMDMs. BMDMs (1.0 × 10⁶ cells · ml⁻¹) from C57BL/6 mice were stimulated with particles at concentrations ranging from 0.4 mg · ml⁻¹ to 1 mg · ml⁻¹ alone or after priming with LPS (10  ng · ml⁻¹) for 3 h. Supernatants were collected 24 hours later and tested for (A) IL-1β secretion by ELISA or (B) LDH release to assess cell viability. (A) Results are mean cytokine concentrations (±SD) for triplicate samples (vs LPS alone NS p > 0.05, *p < 0.05 and ***p < 0.001). Data are representative of three independent experiments. (B) Error bars show means ± SD for triplicate samples (vs media *p < 0.05 and ***p < 0.001). Results are representative of two independent experiments.