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. 2017 Jun 7;7:2932. doi: 10.1038/s41598-017-03279-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Probing the BB0238-BB0323 interaction. (A) Residues 34–67 in BB0238 are not involved in the interaction with BB0323. Yeast two-hybrid assays for assessment of BB0238-BB0323 interaction. The yeast vector pGAD expressing GAL4 activation domain (AD) or various fusions of AD-BB0238 were introduced into yeast along with the vector pGBKT7 expressing the GAL4 DNA binding domain (BD) fused with BB0323. Serially diluted transformants were grown in non-selective (SD-Trp-Leu) or selective (SD–Ade–His–Leu–Trp) media and yeast growth was recorded. (B) BB0323 and BB0238 interact in Far-Western assays. Recombinant BB0323 (left panels) or BB0238 (right panels) was subjected to SDS-PAGE and incubated with glutathione S-transferase (GST)-fused BB0238, or His-tagged BB0323 proteins, and binding was examined by appropriate primary and secondary detection antibodies. (C) Residues 120–130 of BB0238 mediate the interaction with BB0323. Yeast vector expressing indicated AD-BB0238 fusion proteins were cotransformed with BD-BB0323 and growth was assessed, as detailed in panel A. (D) BB0238 missing 11-residue interaction motif failed to bind BB0323 in far-Western assays. Recombinant GST-BB0238 fusion proteins were subjected to SDS-PAGE and incubated with recombinant his-tagged BB0323 and the interaction was monitored as described in panel B. Migration of protein standards is shown to the left in kilodaltons.