Figure 2.

BB0238 interaction region in BB0323 encompasses the 200 N-terminal amino acids. (A) Schematic representation of full-length BB0323. Arrow indicates the predicted signal peptide cleavage site. Residues 226–242 indicate the interaction region between the N- and C-terminal BB0323 polypeptides¹⁷. (B) Yeast two-hybrid assays to identify the BB0238-BB0323 interaction region. Truncated BB0323 fusion proteins, as schematically represented (left panels) were expressed in yeast as BD-BB0323 fusion proteins that were cotransformed with AD-BB0238 and yeast growth was monitored as detailed in Fig. 1A.