Table 1.
The comparison of activity of DAAO with L- and D-amino acid as substrate.
| Substrate | Δ* | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| pH 6.0 | pH 6.5 | pH 7.0 | pH 7.5 | pH 8.0 | pH 8.5 | pH 9.0 | pH 9.4 | pH 9.8 | pH 10.5 | |
| Pro | — | 0.8 | 2.7 | 6.8 | 17.2 | 4.6 | 3.7 | 3.1 | 2.8 | — |
| Ala | — | — | — | — | — | — | — | — | 19.4 | — |
| Arg | — | 11.8 | — | — | — | — | — | — | — | — |
| Phe | — | — | — | — | — | — | 1.1 | — | — | — |
| Val | — | — | — | — | — | 3.0 | — | — | — | — |
| Met | — | — | — | — | — | — | — | — | — | — |
| Ser | — | — | — | — | — | — | — | — | — | — |
| Leu | — | — | — | — | — | — | — | — | 0.6 | — |
| Thr | — | — | — | — | — | — | — | 7.6 | — | — |
| Trp | — | — | — | — | — | — | — | — | — | — |
| His | — | — | — | — | — | — | — | — | - | — |
| Lys | — | — | — | — | — | — | — | — | — | — |
| Cys | — | — | — | — | — | — | — | — | - | — |
| Asn | — | — | — | — | — | 0.6 | — | — | — | — |
| Gln | — | — | — | — | 50 | — | — | — | — | — |
| Asp | — | — | — | — | 77.2 | — | — | — | — | — |
| Glu | — | — | — | — | — | — | — | — | — | — |
Δ* = (OD512(PL-AA)- OD512(Control))/(OD512(PD-AA) − OD512(Control)) × 100, where OD512(PL-AA) and OD512(PD-AA) are the absorbance of the product at 512 nm with L-amino acid and D-amino acid as substrate, respectively; —, no data.