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. 2017 Jun 7;7:2997. doi: 10.1038/s41598-017-02959-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electron microscopy images of B. terrae BS001 wild-type and mutant strains sampled from soft agar. 0.25%, sampled from 2.5 g/L agar; 0.6%, sampled from 6 g/L agar. Wild-type: wild-type BS001 strain; ΔsctD: BS001 ΔsctD mutant strain; ΔpilN: BS001 ΔpilN mutant strain; ΔfliF: BS001 ΔfliF mutant strain. All the flagellum-positive strains (wild-type strain, BS001 ΔsctD mutant strain, BS001 ΔpilN mutant strain) can synthesize flagella on semi-solid agar whereas BS001 ΔfliF mutant strain could not form any flagella.