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. 2017 Jun 7;7:2997. doi: 10.1038/s41598-017-02959-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Flagellar motility of B. terrae BS001 wild-type and mutant strains on soft agar medium. WT, wild-type strain; D, ΔsctD mutant strain; F, ΔfliF mutant strain; N, ΔpilN mutant strain. 0.25%, agar concentration 2.5 g/L; 0.6%, agar concentration 6 g/L. For swimming motility at pH 5.2, the image was obtained following overnight incubation, for the remainder, the pictures were taken 3 days after inoculation. (b) motility of B. terrae BS001 wild-type strain and mutant strains on swarming agar supplemented with 5 mM or 25 mM NaCl. (c) Swimming area (as described by diameter) of B. terrae BS001 wild-type and mutant strains on soft agar (2.5 g/L agar), 5 days after inoculation. (d) Swimming area of B. terrae BS001 wild-type strain on soft agar (2.5 g/L agar) at different pH, 24 h after inoculation. (e) Twitching motility of B. terrae BS001 wild-type and ΔpilN mutant strain (3 days after inoculation). (f) Twitching area of wild-type, ΔpilN mutant and P. aeruginosa PA1 (3 days after inoculation). *P < 0.05, **P < 0.01, ***P < 0.001 compared to wild-type in (c) and (f), compared to pH 5.2 in (d).