Figure 3.

Activation of ERK1/2, p38MAPK and generation of cAMP by H2 relaxin and ML290. In HEK-RXFP1 cells, H2 relaxin activated p-ERK1/2 (A) 5 min), p-p38MAPK (B) 15 min) and cAMP accumulation (C) 30 min) in a concentration-dependent manner. ML290 did not directly activate p-ERK1/2 (A), but did activate p38MAPK (B) with lower efficacy and cAMP accumulation with similar efficacy but significantly lower potency than H2 relaxin (C). 10 min pretreatment with ML290 enhanced p-ERK1/2 activation produced by relaxin (D) 4 min). Data are mean ± SEM for 4–8 independent experiments.