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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Leukemia. 2016 Nov 14;31(6):1415–1422. doi: 10.1038/leu.2016.326

Figure 3. Depletion of Gab2 corrects the hypersensitive growth pattern of Ptpn11E76K/+ myeloid progenitors.

Figure 3

(A) BM cells (2×104 cells/mL) freshly harvested from the mice with the indicated genotypes (n=3/group) were assessed by CFU assays 8 weeks after pI-pC administration with a range of GM-CSF concentrations. (B) BM-derived macrophages were generated from the mice with the indicated genotypes (n=3/group) and cultured in DMEM supplemented with 10% FBS and 20% L cell conditional medium for 3 days. Cell growth rates were determined using a One Solution Cell Proliferation Assay kit. (C) LSK (Lineage−Sca-1+c-Kit+) cells were sorted from the mice with the indicated genotypes (n=6/group) and cultured in the presence of IL-3 (2 ng/mL). After 7 days of in vitro culture, percentages of myeloid (Mac-1+/Gr-1+) cells differentiated from LSK cells were assayed by FACS. Three independent experiments were performed with similar results obtained in each. Data shown are mean±S.D. from one experiment.