. Author manuscript; available in PMC: 2017 Nov 1.

Published in final edited form as: Nat Biotechnol. 2017 May 1;35(6):569–576. doi: 10.1038/nbt.3836

Table 2. Clinical characteristics of patient-derived organoids and outcomes of orthotopic transplantation experiments.

All patient-derived organoid transplantation studies were performed in NSG mice. Tumors were identified by colonoscopy and confirmed by necropsy and histology. For all experiments, two injections were performed per mouse. (T: Tumor; N: Node; M: Metastasis; MSS: microsatellite stable; MSI-H: microsatellite instability-high).

Patient	Age	Sex	Location / pathology	Stage	Mutations	Primary tumors / mice	Liver metastases / primary tumors
Patient	Age	Sex	Location / pathology	Stage	Mutations	Primary tumors / mice	Week 4	Week 8	Week 12	Week 24
A	61	Female	Moderately differentiated liver metastasis from rectal adenocarcinoma	T4 M1	MSS. Mutations in KRAS, TP53, PTCH1	17/18	0/7	1/3	2/4	2/3
B	68	Female	Right colon adenocarcinoma with peritumoral lymphocytic infiltrate	Tis N0 M0	MSI-H, loss of MSH6 nuclear expression on IHC. KRAS wild-type. Mutations in TP53 and PIK3CA	12/13	0/3	1/5	2/4	-
C	47	Female	Moderately differentiated peritoneal metastasis from right colon	T4b N1b M1b	MSS. Mutation in KRAS	11/11	0/4	1/4	1/3	-
All patients (%)	-	-	-	-	-	40/42 (95%)	0/14 (0%)	3/12 (25%	5/11 (45%)	2/3 (67%)