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. Author manuscript; available in PMC: 2017 Jun 19.
Published in final edited form as: Mucosal Immunol. 2016 Dec 7;10(4):1031–1042. doi: 10.1038/mi.2016.105

Figure 2. Memory-like NK cells expand after BCG vaccination and challenge with M. tb H37Rv.

Figure 2

C57BL/6 mice (20 mice per group) were given 100 µl of PBS or immunized subcutaneously with 106 CFU of BCG in 100 µl of PBS. After thirty days, mice were challenged with 75–100 CFU of M. tb H37Rv by aerosol. At weekly intervals up to 4 weeks, five mice in each group were sacrificed, and the lung bacterial burden and percentages of CD3-NKp46+ cells in lungs and spleen that were CD27+ were determined. (A) CD3-NKp46+CD27+ cells in lungs. (B) CD3-NKp46+CD27+ cells in spleens. (C) A representative flow cytometry plot is shown. Gating strategy to identify NK cells was similar to Figure 1. (D) Bacterial burden in lungs. Mean values and SEs are shown. Data are representative of two independent experiments.