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. 2017 Jun 8;8:348. doi: 10.3389/fphys.2017.00348

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Copyright © 2017 Messer, Chan, Daley, Copeland, Marston and Connolly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulation of Ca²⁺-sensitivity by TnI phosphorylation in non-HCM and HCM cat. Sliding speed (top graphs) and fraction of filaments motile (bottom graphs) was measured with phosphorylated and unphosphorylated troponin by IVMA. Ca²⁺ dependence of motility is plotted against [Ca²⁺] for representative single experiments. (A) Phosphorylated and unphosphorylated non-HCM cat troponin (N4), (B) phosphorylated and unphosphorylated HCM cat troponin (H5). The Hill equation is fitted to the data to yield values of EC₅₀ and n_H Solid lines, phosphorylated; dotted lines, unphosphorylated. The mean ratios of EC₅₀ values from replicate experiments is shown in Figure 6 and Table 3. Full data is shown in Supplementary Table S3.