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. 2017 Jun 8;8:348. doi: 10.3389/fphys.2017.00348

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Copyright © 2017 Messer, Chan, Daley, Copeland, Marston and Connolly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recoupling of HCM cat thin filaments by EGCG. (A) The effect of 100 μM EGCG on cat HCM sample H5 measured by IVMA. The Hill equation is fitted to the data to yield values of EC₅₀ and n_H. Solid symbols and lines, phosphorylated; open symbols and dotted lines, unphosphorylated. Red points and lines are in the absence and green points and lines are in the presence of EGCG. EGCG reduces the Ca²⁺-sensitivity of phosphorylated but not unphosphorylated H5 troponin and thus restores coupling. (B) Demonstration of complete restoration of coupling by EGCG. The figure shows the phosphorylated and unphosphorylated EC₅₀ of the non-HCM cat sample N12 (coupled) and the HCM cat sample H13 (uncoupled). Addition of EGCG to H13 restores phosphorylation-dependence of EC₅₀ to H13. Full data in Supplementary Table S5.