FIGURE 4.

mRNA and protein expression in L6 myotubes on pretreatment of quercetin. (A) mRNA levels in L6 myotubes on pretreatment. The bar graph shows the mRNA levels (mean ± SE) of GLUT 4, CaMKK, AMPK, MAPK, PI3K, Akt and Irs in L6 myotubes on pretreatment of quercetin. The mRNA levels (arbitrary units) are expressed in relative to those of control cells. Quercetin (10 and 100 μM):Qn2 and Qn3. Significance test between different groups were determined by using one way ANOVA followed by Duncan’s multiple range test the significance accepted at P ≤ 0.05. ^∗P ≤ 0.05 versus Untreated Control. (B) Protein expression in L6 myotubes. The effect of quercetin (10 and 100 μM): Qn2 and Qn3 on pretreatment for 24 h was comparable with that of the positive control rosiglitazone (100 nM). Significance test between different groups were determined by using one way ANOVA followed by Duncan’s multiple range test the significance accepted at P ≤ 0.05. ^∗P ≤ 0.05 verses untreated control.