Table 3.
List of specific primers designed for the PCR-based identification of X. fastidiosa.
| Forward primer | Reverse primer | Size | Target | Hosts | Citations WoS - G-Scholar | References |
|---|---|---|---|---|---|---|
| RST31: GCGTTAATTTTCGAAGTGATTCGA | RST33: CACCATTCGTATCCCGGTG | 733 | 7.4 kb EcoR1 restriction fragment | Grapevine, citrus, oak, red oak, sycamore, plum, goldenrod | 193–285 | Minsavage et al., 1994 |
| XF1-F: CAGCACATTGGTAGTAATAC | XF6_R: ACTAGGTATTAACCAATTGC | 400 | 16s rDNA | Grapevine, almond, plum, American elm, citrus | (nd 2004) – 16 | Firrao and Bazzi, 1994 |
| 208-1: ACGGCCGACCATTACTGCTG | 208-2: ACGGCCGACCCGGAGTATCA | 320 | RAPD fragment | Citrus, grapevine, mulberry, almond, plum, elm, oak, ragweed, periwinkle | 119–180 | Pooler and Hartung, 1995a |
| 203-1: CACGGCGAGTATCGGCTTTC | 203-2: CACGGCGAGTCGACGTAAAT | 2,000 | – | – | ||
| 204-1: TTCGGGCCGTCAATGTGTTG | 204-2: TTCGGGCCGTTTACTCAAAG | 800 | – | – | ||
| 230-1: CGTCGCCCATCAACGCCAAA | 230-2: CGTCGCCCATTGTGCTGCAG | 700 | – | – | ||
| 272-1: AGCGGGCCAATATTCAATTGC | 272-2: AGCGGGCCAAAACGATGCGTG | 700 | – | – | ||
| 272-1-int: CTGCACTTACCCAATGCATCG | 272-2-int: GCCGCTTCGGAGAGCATTCCT | 500 | – | – | ||
| CVC-1: AGATGAAAACAATCATCGAAA | 272-2: AGCGGGCCAAAACGATGCGTG | 600 | – | Citrus | ||
| CVC-1: AGATGAAAACAATCATCGAAA | 272-2-int: GCCGCTTCGGAGAGCATTCCT | 500 | – | Citrus (specific) | ||
| XF176f: AAACAATCACAGGGGACTGC | XF954r: CTATCCGAACACTTCCTATG | 779 | PD-specific RAPD fragment (PD1-1-2) | Grapevine (Pierce's disease) | 20–29 | Banks et al., 1999 |
| XF176f: AAACAATCACAGGGGACTGC | XF686r: ATATTCATAGATTCCGTCGA | 511 | – | Citrus, mulberry, oak, periwinkle, peach, plum (Non-Pierce's disease) | ||
| 0067-a-S-19: CGGCAGCACATTGGTAGTA | 1439-a-A-19: CTCCTCGCGGTTAAGCTAC | 1,348 | 16S rDNA | Citrus, grapevine, mulberry | 43–70 | Rodrigues et al., 2003 |
| 0067-a-S-19: CGGCAGCACATTGGTAGTA | 0838-a-A-21: CGATACTGAGTGCCAATTTGC | 745 | – | – | ||
| 0838-a-S-21: GCAAATTGGCACTCAGTATCG | 1439-a-A-19: CTCCTCGCGGTTAAGCTAC | 603 | – | – | ||
| FXYgyr499: CAGTTAGGGGTGTCAGCG | RXYgyr907: CTCAATGTAATTACCCAAGGT | 429 | b-subunit polypeptide of the DNA gyrase (gyrB) | – | ||
| G1: GAAGTCGTAACAAGG | L1: CAAGGCATCCACCGT | 522 | 16S rDNA | Porcelain berry, wild grape, Mulberry | Huang and Sherald, 2004 | |
| Teme150fc: TCTACCTTATCGTGGGGGAC | Teme454rg: ACAACTAGGTATTAACCAATTGCC | 348 | 16S rDNA | Almond | 54–78 | Chen et al., 2005 |
| Teme150fc: TCTACCTTATCGTGGGGGAC | Xf16s1031r: AAGGCACCAATCCATCTCTG | 700 | – | – | ||
| Dixon454fa: CCTTTTGTTGGGGAAGAAAA | Dixon1261rg: TAGCTCACCCTCGCGAGATC | 847 | – | – | ||
| REP1-R: IIIICGICGIATCCIGGC | Xf-1: CGGGGGTGTAGGAGGGGTTGT | 350 | Amplified genomic fragment | Citrus, grape, almond, mulberry, oak, periwrinkle, plum, coffee, elm, ragweed | 3–7 | Travensolo et al., 2005 |
| XF1968-L: GGAGGTTTACCGAAGACAGAT | XF1968-R: ATCCACAGTAAAACCACATGC | 638 | Gene XF1968 | Almond, oleander | 21–42 | Hernandez-Martinez et al., 2006 |
| XF2542-L:TTGATCGAGCTGATGATCG | XF2542-R: CAGTACAGCCTGCTGGAGTTA | 412 | Gene XF2542 | Grape, almond, Spanish broom, Brassica spp. | ||
| ALM1: CTGCAGAAATTGGAAACTTCAG | ALM2: GCCACACGTGATCTATGAA | 521 | Gene ALM1 | Almond (specific) | ||
| 16S-23SF: GATGACTGGGGTGAAGTCGT | 16S-23SR: GACACTTTTCGCAGGCTACC | 650 | 16S-23S intergenic spacer | Citrus, coffee, grapevine, mulberry, almond elm, ragweed, periwinkle | 1–2 | Martinati et al., 2007 |
| 307 BBF: GCAAGTCAGGGTAGCGTCTC | 943 BBR: GGCTTCTCTGTCGATTTTCG | nd | mopB | Grape, Sea myrtle, Redspike Mexican hat, others | 5–10 | Morano et al., 2008 |
| HQ-OLS08: TGTACGTCCTGAAACCATCTTG | HQ-OLS05: TTCTGGAAGCTTTGAGTAAGGG | 274 | RAPD fragment | Oleander (specific) | 7–10 | Huang, 2009 |
| D056L: AACAAGGGACCTTCCATGC | D858R: AGCAATCGCTGCACCTAAAT | 842 | Succinyl-CoA synthetase alpha subunit (sucD) | Almond | 0–0 | Livingston et al., 2010 |
| G151f: GATCCGGAAAGTGGGGAGATTACTATC | G368r: GCCATTGCAAAAGCAGTACGCTCA | 217 | DNA polymerase III subunit beta (dnaN) | – | ||
| A151f: GATGCGGAAAGCGGGGAGATTACTATT | A518r: GCCTTTACGCGGCAAAATAATCTGA | 367 | DNA polymerase III subunit beta (dnaN) | – | ||
| A97f: CATGCTGGTGGTAAGTTCGACGATAAC | A476r: CAACAATGCCGTTGTGCTCACCG | 379 | b-subunit polypeptide of the DNA gyrase (gyrB) | – | ||
| G223f: CGGTGGCGAAACGGTAATCC | G705r: GGAGAAATGTTTGGCAAAGACAGGC | 482 | mdh Malate dehydrogenase | – | ||
| A345f: TTTTTCAATGTTGGCGACAGGCTTACT | A705r: GGAGAAATGTTTGGCAAAGACAGGT | 360 | mdh Malate dehydrogenase | – | ||
| B001L: TTAGGTGGCAAGGATCGAAT | B462R: GGGCCGATCAAAATCAATCT | 491 | ppiB Peptidyl-prolyl cis-trans isomerase | – | ||
| A284f: GACGAGTTTGCCAAGTTTGATGATGAAATC | A680r: GCCAGTCGAACCCACCAAG | 396 | gltA Citrate synthase | – | ||
| I068L: CGTGGGTCACGAGTCATAAA | I386R: TCACACAAAACTACGGCACTG | 358 | rpsI 30S ribosomal protein S9 | – | ||
| PspB-256f: TGAGTGCCTGCGGTGGTA | PspB-256r: CGAAACTTGGCAGCTAACG | 256 | pspB Serine protease | – | ||
| XFPglA_Fw: GCCTCCGGTGCGACTGCTTC | XFPglA_Rv: GCTGCGATTGGACACACATTG | nd | PglA | Pecan, Grapevine, Oleander, sycamore | 10–14 | Melanson et al., 2012 |
| Mul-15040-F: ATTTTCGCGATTTTGGAGTT | Mul-15040-R: TTCTTGTGTACTCCGCCTCA | 312 | Hypothetical protein with putative bacillithiol system oxidoreductase, YpdA family | Mulberry and olive (specific) | 2–2 | Guan et al., 2015 |
| Xfa-rpod-F4: ACTGAGGTTGTCGTTGGCTT | Xfa-rpod-R4: CCTCAGGCATGTCCATTTCC | 988 | RNA polymerase sigma-70 factor (rpoD) | Olive, citrus, coffee | 1–2 | Bleve et al., 2016 |
| Xfa-dnaA-2F: TTCCATCAAATTGACGCGCT | Xfa-dnaA-2R: CGGCAAGCATGTAACACTGT | 650 | Chromosomal replication initiator protein DnaA (dnaA) | – |