Figure 3.

The enhancement of memory CD8 T cells by pAIM2/pVP1 co-immunization. 16 weeks after the last immunization, spleen cells from each immunization groups (n = 8 per group) were harvested and analyzed by immunofluorescence staining and flow cytometry. (A) The frequency of CD8 T cells showing memory subset phenotypes: naive cells (CD44⁻CD62L^high), effector memory (CD44⁺CD62L^low), and central memory (CD44⁺CD62L^high) in spleen from various vaccine immunized mice. One representative flow cytometry result was shown for per group. (B) Statistical analysis of the absolute number of naive, effector memory, and central memory cells in spleen. Data are from one experiment of three independent experiments and presented as the mean ± SD. ^*p < 0.05, ^**p < 0.01.