FIGURE 4.
Interaction between ABA and JA in rice roots. (A–F) Hormone levels (ng/g of fresh weight) in root tissue of rice plants at 24 h after foliar application of 50 and 200 μM ABA or abamine and corresponding roots of water-treated control plants. (A,B) ABA levels (C,D) SA levels (E,F) JA levels. Bars show the mean and standard error of mean of five biological replicates. (G) Effect of application of exogenous JA (MeJA, 100 μM), ABA (50 μM) or both, as well as PBX::AOS2-overexpression (AOS2-OX), alone or in combination with ABA (50 μM) on rice susceptibility for the root knot nematode M. graminicola. At 24 h after foliar chemical treatment, plant roots were inoculated with 250 second stage juveniles of M. graminicola. Bars represent the mean (n = 8) and standard error of mean as counted at 14 days after inoculation. Data represent one of three independent experiments with similar results. Different letters indicate statistically significant differences (Duncan’s multiple range test with α = 0.05). ABA, abscisic acid; SA, salicylic acid; JA, jasmonic acid; AOS, allene oxide synthase.