Figure 10.

Effect of repeated in vivo IRI administration on the density of VAChT-IR cholinergic fibers in the myenteric plexus. Wholemount preparations of VAChT-IR (green) fibers in the myenteric ganglia of the colon from sham and IRI-treated mice at 14 days (A,A′) and 7 days post-treatment (B,B′) Scale bar = 25 μm. (C) Density of VAChT-IR fibers in the myenteric plexus was analyzed at 14 days and post-treatment in both sham and IRI-treated mice. Gray columns: sham-treated, black columns: IRI-treated mice. Data presented as mean ± S.E.M. ^**P < 0.01, ^****P < 0.0001, n = 5 mice/group/time point.