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. 2017 Jun 8;10:179. doi: 10.3389/fnmol.2017.00179

Figure 3.

Figure 3

Neuritin suppresses NEURL1-mediated JAG1 endocytosis and Notch signaling. (A,B) Neuritin overexpression suppressed NEURL1-mediated JAG1 endocytosis and degradation in 293T cells expressing HA-JAG1. Cells were transfected with Flag-NEURL1 only or with Flag-NEURL1 and His-neuritin. JAG1 endocytosis was visualized by immunofluorescence. Corresponding experiments show expression levels of HA-JAG1 and other cellular proteins by western blotting analysis and densitometry. *p < 0.01, 2-sided Fisher’s exact test. Scale bar represents 10 μm. (C,D) Knockdown of neuritin increased NEURL1-mediated JAG1 endocytosis and degradation in 293T cells expressing HA-JAG1 and Flag-NEURL1 transfected with or without neuritin siRNA-3. JAG1 endocytosis was visualized by immunofluorescence. Protein expression in transfected cells was detected by western blotting and densitometry. *p < 0.01, 2-sided Fisher’s exact test. Scale bar represents 10 μm. (E) Expression of Flag-NEURL1 in 293T cells transfected with or without His-neuritin. The NICD and HES1 were detected by western blotting analysis and densitometry. pcDNA3.1 empty vector was used as a control. *p < 0.01, 2-sided Fisher’s exact test.