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. 2017 Jun 8;8:989. doi: 10.3389/fpls.2017.00989

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Copyright © 2017 Kang, Kim, Kwon, Ke, Ji, Park, Lee, Deng and Kwak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IbPsbP interacts with IbOr. (A) Bimolecular fluorescence complementation (BiFC) assays for in planta interaction of IbPsbP with IbOr in chloroplasts. N. benthamiana leaves were transformed by Agrobacterium harboring N-terminal region of Venus (NV) and C-terminal region of Venus (CV) construct pairs and observed by confocal laser scanning microscopy. BR, bright field microscopy images; CHL, chlorophyll autofluorescence; VENUS, Venus fluorescence images; MERGE, overlay of bright field, chlorophyll, and Venus images. Scale bar = 20 μm. (B) Pull-down assay for in vitro interaction of IbOr with IbPsbP. Gels containing pull-down assay products were immunoblotted with anti-His. His-IbOr, GST (negative control), and GST-IbPsbP proteins are shown in the indicated combinations.