FIGURE 4.

MiR-590 directly inhibits VEGF expression by targeting its mRNA 3′-UTR. (A) Sequences of the predicted miR-590 targeting sites on the 3′-UTR of VEGF mRNA. (B) Wild type (LF-wt-VEGF) or mutated (LF-mut-VEGF) sequences from VEGF mRNA 3′-UTR were cloned to the 3′-prime of luciferase reporter gene (LF). (C) Luciferase activities of LF-wt and LF-mut constructs were assessed in NSCs expressing control or miR-590. (D) VEGF protein expression was examined in NSCs expressing control or miR-590. Data were presented as mean ± SD from at least three independent experiments. ^∗∗p < 0.01, ns not significant, vs. control.