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. 2017 Jun 8;10:183. doi: 10.3389/fnmol.2017.00183

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Copyright © 2017 Li, Wang, Li and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-590 expression in NSCs is repressed by hypoxia via HRE in its promoter region. (A) Sequences of the predicted HRE in the promoter region of miR-590 (Wt-HRE-LF) and its mutated version (Mut-HRE-LF) were cloned to the 5′-prime of luciferase reporter gene (LF). (B) Isolated NSCs were treated with either 0 (normoxia) or 100 μM CoCl₂ (hypoxia), respectively, followed by ChIP assay using control IgG or HIF-1α antibody (Ab). (C) Luciferase activities of Wt-HRE-LF or Mut-HRE-LF constructs described in (A) were assessed in NSCs under normoxia or hypoxia conditions, respectively. (D) Expressions of miR-590 was analyzed in NSCs under normoxia or hypoxia conditions, respectively. Data were presented as mean ± SD from at least three independent experiments. ^∗∗p < 0.01, ns not significant, vs. control IgG or normoxia.