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. 2017 Jun 8;8:965. doi: 10.3389/fpls.2017.00965

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Copyright © 2017 Chen, Liu, Lou and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein sequence alignment and phylogenetic tree of MaAN2 transcription factor and its putative orthologs, and tissue-specific analysis of MaAN2. (A) Alignment of the deduced amino acid sequences of MaAN2 and other R2R3-MYBs associated with flavonoid biosynthesis from other plant species. If the identities of amino acids in each column are 100, 75, and 50%, these amino acids are indicated on a black, dark gray, and light gray background, respectively. The R2 and R3 repeats are indicated above the alignment. The motif [D/E]LX₂[K/R]X₃LX₆LX₃R in the R3 repeat necessary for interaction with a bHLH protein in the R3 repeat is indicated by dark triangles. The small motif [K/R]P[Q/R]P[Q/R] in the AN2 subgroup is shown by a red box. The short conserved sequences KAX[K/R]C[S/T], recognized in the C1 subgroup, are shown by blue box. (B) The maximum-likelihood phylogenetic tree was generated using MEGA 6.0 software. Numbers next to the nodes indicate the bootstrap values from 1000 replications. The bar indicates a genetic distance of 0.1. The gene names from various plant species and NCBI GenBank accession numbers for the sequences are as follows: Arabidopsis thaliana AtPAP1 (NP_176057), AtPAP2 (NP_176813); Petunia hybrida PhAN2 (AAF66727); Nicotiana tabacum NtAN2 (ACO52472); Solanum lycopersicum SlMYB (AAQ55181); Antirrhinum majus AmROSEA1 (ABB83826), AmVENOSA (ABB83828)); Ipomoea nil InMYB1 (BAE94391.1); Gerbera hybrida GMYB10 (CAD87010); Vitis vinifera VvMYBA1 (BAD18977); Malus domestica MdMYB1 (ABK58138) and MdMYB10 (ABB84753); Malus crabapple McMYB10 (AFP89357); Epimedium sagittatum EsAN2 (ALO24362); Solanum tuberosum StAN1-R1 (AKA95392); Elaeis guineensis EgVIR (KJ789862); Allium cepa AcMYB1 (KX785130); Anthurium andraeanum AaMYB2 (KU726561); Lilium hybrid division I LhMYB6 (BAJ05399) and LhMYB12 (BAJ05398); Gentiana triflora GtMYB3 (BAF96933); Oncidium Gower Ramsey OgMYB1 (ABS58501); Zea mays ZmPl (NP_001105885) and ZmC1 (NP_001106010); Oryza sativa OsMYB (CAA75509); Phalaenopsis schilleriana PsUMYB6 (ACH95792); Phalaenopsis equestris PeMYB2 (AIS35919), PeMYB11 (AIS35928), and PeMYB12 (AIS35929); Dendrobium hybrid DhMYB2 (AQS79852); Human cMYB (M15024). (C) The expression profile of MaAN2 in the roots, bulbs, leaves, and flowers of Muscari armeniacum.