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. 2017 Jun 8;8:965. doi: 10.3389/fpls.2017.00965

FIGURE 4.

FIGURE 4

Schematics of M. armeniacum anthocyanin biosynthetic gene promoters and dual luciferase transient expression assay. (A) The lengths, MYB binding sites, and bHLH binding sites of MaCHS, MaCHI, MaF3H, MaDFR, and MaANS promoters are marked by black solid lines, red circles, and blue rectangles, respectively. (B) Dual luciferase transient expression assay showed the effects of MaAN2, AtPAP1, and AtTT8 TFs on MaCHS, MaCHI, MaF3H, MaDFR, MaANS, and A. thaliana AtDFR promoters. All TFs were co-infiltrated with promoters in N. benthamiana leaves for transient transformation assays. The results show the promoter activity expressed as the ratio of the activities of these promoters: luciferase (LUC) to 35S: Renilla (REN). Each column represents means ± SD from four biological replicates.