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. 2017 Jun 8;8:1027. doi: 10.3389/fmicb.2017.01027

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Copyright © 2017 Hassan, Maldonado, dos Santos, Di Lorenzo, Silipo, Coutinho, Cooper, Molinaro, Valvano and Sá-Correia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A), including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis (Carver et al., 2008)], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 (Bc IST439), B. multivorans IST419 (Bm IST419), and B. multivorans ATCC 17616 (Bm ATCC17616).