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. 2017 Jun 8;8:1027. doi: 10.3389/fmicb.2017.01027

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Copyright © 2017 Hassan, Maldonado, dos Santos, Di Lorenzo, Silipo, Coutinho, Cooper, Molinaro, Valvano and Sá-Correia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the OAg unit after cloning and introduction of B. cenocepacia IST439 wbiI and Bmul_2510 genes in all variants lacking OAg production to complement the corresponding mutated genotypes. (A) LPS electrophoretic profiles for IST439, IST4134, and IST4103 showing the presence/absence of OAg units, and OAg absence in all transconjugants selected; (+) denotes 1% (w/v) rhamnose and (–) denotes no rhamnose added to the medium. (B) Western blots showing bands at around 60 KDa (left) and 75 KDa (right), which indicate WbiI and Bmul_2510 polypeptides, respectively. Lanes shown (left to right): protein ladder (M), cloning vector pSCrhaB2, cloning vector + WbiI-FLAG or Bmul_2510-FLAG.